MolBioCove

CORE CAPABILITY

Sequence Workspace

Edit sequences with the context you need—features, overlays, and annotations tied to the underlying sequence. Design primers, plan digests, and stage cloning workflows without losing provenance.

PRODUCT VIEW

Sequence Workspace in action

Overlays (Protein, ORFs, Restriction, Primers) stay tied to the sequence—so you immediately see what a change affects.

MolBioCove sequence workspace screenshot

WORKSPACE TOOLS

Primers, digests, and cloning—without leaving the sequence

MolBioCove Primer Wizard showing modes and primer constraints

Primer Wizard

Generate primers from any selection with constraints you control—and keep them tied to the exact sequence context they were designed against.

  • Single, qPCR, cloning, and QuikChange-style mutagenesis modes
  • Tune length, Tm, and GC% with practical guardrails (including a GC clamp)
  • Optional off-target checks and mismatch handling
  • Add 5′ tails for restriction sites, Golden Gate, Gibson, or custom adapters
Primer design panel showing a restriction enzyme site added to a primer

Add restriction sites in one click

When you already know the enzyme, MolBioCove adds the site plus 5′ flanking bases and shows an order-ready sequence.

  • Choose an enzyme and set 5′ flanking bases before the site
  • See length, Tm, and GC% update live as you edit
  • Copy a clean 5′→3′ sequence for ordering
Digest planner showing enzyme selection, fragment list, and virtual gel preview

Digest planning with virtual gel preview

Choose enzymes, inspect predicted fragments, and preview a virtual gel—so you pick the right backbone and inserts before you cut DNA.

  • Combined and single-enzyme digest modes
  • Fragment table with start/end coordinates and feature context
  • Copy fragments or export CSV
  • Virtual gel preview for a quick sanity check
Cloning wizard showing staged inserts, Gibson settings, and simulation controls

Cloning wizard + simulation

Stage a backbone and inserts, simulate the assembly, and export a report for your notebook—or a clean handoff.

  • Technique recommendation (e.g., Gibson) with a confidence hint
  • Auto-sized overlaps plus minimum overlap length and Tm controls
  • Simulate before committing to a build
  • Export a cloning report (PDF)
Sequence view with protein translation overlay and selection context

Protein & ORF context while you edit

Translate in-frame, keep amino-acid context visible, and tie AA/codon indexing to the underlying sequence—so protein edits stay deliberate.

  • In-frame amino-acid track under the sequence
  • Clear AA/codon indexing on hover and selection
  • Overlays for ORFs, restriction sites, primers, and features
Tooltip showing amino-acid and codon indexing for a selection

Hover any selection to see amino-acid and codon indexing.

Dialog prompting to add a primer to lab inventory with location and position

Link primers to physical inventory

Link primers (and plasmids) to where they live—so the right tube is always findable.

  • Store location, container, and position (e.g., A1)
  • Optional prompt right after primer creation
  • Cleaner handoffs and fewer “which tube is this?” moments

WHAT YOU CAN DO

Common tasks

TYPICAL WORKFLOW

A typical flow

Open

Open a construct and bring sequence context, features, and overlays into view.

Edit

Make edits while keeping annotations and protein/ORF context visible.

Plan

Design primers, plan digests, and sanity-check cloning steps before you commit to a build.

Share

Export primer sequences, fragment lists, and a cloning report—so handoffs stay reviewable.

OUTPUTS

Outputs

Request Beta Access

Join the external beta—priority given to active bench teams.

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