Design workflow
Plan a site-directed change in construct context.
For researchers starting with a supported plasmid and a defined target region. Select the region, make or plan the change, review mutagenesis primer candidates, and carry the resulting construct into sequencing review.
Input, steps, and result
- Input: a GenBank, FASTA, or imported SnapGene
.dnaconstruct and a known target region. - Select or edit the target in sequence view with coding context visible.
- Open primer design, choose the appropriate mode, and compare candidates and diagnostic flags.
- Keep or export the chosen primer records, then use sequencing review after the experiment.
- Result: a documented primer choice and edited construct context ready for validation.
Limitations: automated primer candidate generation is a Pro capability. Experimental outcome is not predicted or guaranteed; confirm primers and the final construct with appropriate laboratory review.

Keep your construct and its evidence in one Mac workflow.
Download RayCrest MDS for Mac. RayCrest Pro is $9.99/month.