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Design workflow

Plan a site-directed change in construct context.

For researchers starting with a supported plasmid and a defined target region. Select the region, make or plan the change, review mutagenesis primer candidates, and carry the resulting construct into sequencing review.

Input, steps, and result

  1. Input: a GenBank, FASTA, or imported SnapGene .dna construct and a known target region.
  2. Select or edit the target in sequence view with coding context visible.
  3. Open primer design, choose the appropriate mode, and compare candidates and diagnostic flags.
  4. Keep or export the chosen primer records, then use sequencing review after the experiment.
  5. Result: a documented primer choice and edited construct context ready for validation.
Limitations: automated primer candidate generation is a Pro capability. Experimental outcome is not predicted or guaranteed; confirm primers and the final construct with appropriate laboratory review.
Mutagenesis primer candidates displayed for review

Next: review sequencing evidence ยท See pricing

Keep your construct and its evidence in one Mac workflow.

Download RayCrest MDS for Mac. RayCrest Pro is $9.99/month.