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Cloning workflow

Stage the assembly, inspect the junctions, create the construct.

For researchers starting from a backbone and reusable insert or fragment records. The repository documents a Gibson workflow from staging through overlap QC and final construct creation.

A documented planning sequence

Input: a backbone plus one or more inserts or fragments available in the workspace.

  1. Switch to cloning view and stage the assembly.
  2. Arrange fragments in the intended order.
  3. Inspect overlap and junction quality.
  4. Create the assembled plasmid record.

Result: a new in-silico construct ready for review and map export.

Gibson cloning documentation

Gibson assembly staged with backbone and insert fragments
Limitations: simulation supports planning and review; it does not replace experimental controls or confirm that a physical assembly succeeded. Cloning simulation is a Pro capability.

Keep your construct and its evidence in one Mac workflow.

Download RayCrest MDS for Mac. RayCrest Pro is $9.99/month.