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Validation workflow

Review the evidence before accepting the construct.

For scientists comparing sequencing output with an intended construct. Import one evidence type, inspect mismatches and consensus context, and record the QC decision supported by the displayed evidence.

From run to decision

Input: Sanger .ab1/.abi traces or FASTQ files and the reference construct.

  1. Import one sequencing modality at a time.
  2. Review alignment processing and flagged positions.
  3. Open the evidence at the selected mismatch.
  4. Inspect chromatogram, consensus, and coding context where available.

Result: a construct-linked QC review, not an unsupported claim that every sample passed.

Detailed AB1 documentation

Sanger trace peaks aligned with a selected mismatch
Limitations: Sanger and FASTQ files cannot be mixed in one import selection. Automated sequencing-QC interpretation and coding-impact analysis are Pro capabilities; inspect evidence and apply scientific judgment.

Keep your construct and its evidence in one Mac workflow.

Download RayCrest MDS for Mac. RayCrest Pro is $9.99/month.