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C1V1 dilution

C1V1 Dilution Calculator — Stock Volume and Diluent

Calculate how much stock and diluent to combine for a lower target concentration and final volume.

Calculations run locally in your browser. Values are only placed in a URL when you choose “Copy Shareable Link.”

Dilution inputs

Examples

Worked example

Diluting 1 M stock to 100 mM with a 50 mL final volume requires 5 mL stock and 45 mL diluent—a 10× dilution.

How to use

  1. Enter C1, C2, and V2 with their units.
  2. Calculate V1 and diluent.
  3. Review the bench-ready instruction and copy the complete summary.

Validation and limitations

All inputs must be positive, and C2 cannot exceed C1. The tool will not present a concentration-up operation as a dilution recipe. Account for volume non-additivity when your chemistry requires it.

Calculation record

Methodology and scientific authority

Calculate stock and diluent volumes for a concentration decrease using C1V1 = C2V2.

Engine
dilution-plan 1.0.0
Content updated

Methodology

Normalizes concentration and volume units, solves C1V1 = C2V2 for stock volume, and subtracts stock volume from final volume for diluent.

Inputs

  • stock concentration
  • target concentration
  • final volume

Outputs

  • stock volume
  • diluent volume
  • dilution factor
  • bench instruction

Validation examples

These scientific fixtures are reproducible tests of the calculation engine, not build-only checks.

  • 1 M to 100 mM at 50 mL yields 5 mL stock and 45 mL diluent. Test: tenfold-dilution

Assumptions and limitations

Assumptions

  • The amount of solute is conserved through the ideal dilution.

Limitations

  • The tool rejects concentration-up requests.
  • Volume non-additivity and chemistry-specific handling are outside the model.

Save recipes and run timed protocols.

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