Worked example
Resuspending 20 nmol at 100 µM requires 200 µL. A 1 mL, 10 µM working stock then uses 100 µL stock and 900 µL diluent.
DNA & RNA
Oligo Resuspension & Working-Stock Calculator
Resuspend a dried DNA or RNA oligo, prepare a lower-concentration working stock, or connect both steps in one bench-ready record.
Calculations run locally in your browser. Values are only placed in a URL when you choose “Copy Shareable Link.”
How to use
- Select one or both workflow steps.
- Enter the supplied amount and desired molar concentrations.
- Review and copy the complete resuspension and dilution instructions.
Assumptions
The volume equation uses molar amount and concentration; DNA versus RNA is recorded descriptively. The tool does not infer purity, modifications, or a preferred diluent.
Validation and limitations
All required values must be positive. Concentration-up requests are rejected. Small-volume and unusually large-volume messages are planning warnings—not universal pipetting or storage rules.
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