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DNA & RNA

Oligo Resuspension & Working-Stock Calculator

Resuspend a dried DNA or RNA oligo, prepare a lower-concentration working stock, or connect both steps in one bench-ready record.

Calculations run locally in your browser. Values are only placed in a URL when you choose “Copy Shareable Link.”

Oligo plan

Resuspend Dried Oligo
Prepare Working Stock

Worked example

Resuspending 20 nmol at 100 µM requires 200 µL. A 1 mL, 10 µM working stock then uses 100 µL stock and 900 µL diluent.

How to use

  1. Select one or both workflow steps.
  2. Enter the supplied amount and desired molar concentrations.
  3. Review and copy the complete resuspension and dilution instructions.

Assumptions

The volume equation uses molar amount and concentration; DNA versus RNA is recorded descriptively. The tool does not infer purity, modifications, or a preferred diluent.

Validation and limitations

All required values must be positive. Concentration-up requests are rejected. Small-volume and unusually large-volume messages are planning warnings—not universal pipetting or storage rules.

Calculation record

Methodology and scientific authority

Resuspend a dried oligo and optionally prepare a connected lower-concentration working stock.

Engine
oligo-resuspension 1.0.0
Content updated

Methodology

Uses volume = molar amount ÷ molar concentration for resuspension and C1V1 = C2V2 for an optional working dilution.

Inputs

  • oligo amount
  • stock concentration
  • working concentration and volume
  • diluent labels

Outputs

  • resuspension volume
  • stock and diluent volumes
  • dilution factor
  • bench instructions

Validation examples

These scientific fixtures are reproducible tests of the calculation engine, not build-only checks.

  • 20 nmol at 100 µM requires 200 µL. Test: twenty-nmol
  • 100 µM to 10 µM at 1 mL uses 100 µL stock plus 900 µL diluent. Test: working-stock-tenfold

Assumptions and limitations

Assumptions

  • DNA versus RNA and diluent choice are descriptive and do not change the molar volume equation.

Limitations

  • The calculation does not infer synthesis yield, purity, modifications, extinction coefficient, storage stability, or a universally preferred diluent.

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